Figure 3.

Assignment procedure for [5‐¹⁹F,5‐¹³C]‐uridine resonances by diluted site‐specific stable isotope labeling. a) Secondary structure representation of the hHBV ϵ RNA with uridines highlighted in green. The [6‐D, 5‐¹⁹F,5‐¹³C]‐ and [6‐D, 5‐¹⁹F]‐uridine labels are shown. b–f) ¹⁹F–¹³C‐TROSY of site‐specifically labeled RNAs. The [6‐D, 5‐¹⁹F,5‐¹³C] labeled Us are given, all other Us were [6‐D, 5‐¹⁹F] labeled. Fold specific ¹⁹F chemical shift regions (A–U Watson–Crick base paired bp, single stranded ss, and G–U wobble base pairs gu) are marked with dashed boxes.