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. 2020 Sep 7;80(15):1328–1340. doi: 10.1002/pros.24060

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© 2020 The Authors. The Prostate published by Wiley Periodicals LLC

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Shh‐Gli1 signaling is required for ISO‐mediated proliferation of PCa cells. A, Colony formation assays and quantification of cells treated with DMSO, GANT61 (10 μM), or ISO (1 μM) in the absence or presence of GANT61 (10 μM). Data represent the mean ± SD of three experiments. *P < .05. B and C, Western blotting and relative quantification of mRNA expression of Gli1 in PC‐3 and LNCaP cells transduced with a control shRNA (shNC) and shRNA targeting Gli1 (shGli1). D, Colony formation assays and quantification of transduced cells targeting Gli1 without or with 1 μM ISO treatment. β₂‐AR, β₂‐adrenergic receptor; DMSO, dimethyl sulfoxide; ISO, isoproterenol; mRNA, messenger RNA; SD, standard deviation; shRNA, small hairpin RNA [Color figure can be viewed at wileyonlinelibrary.com]