Table 1.

Antibodies’ references and immunohistochemistry and Western blot analysis procedures

Antibodies	Reference	Type	Dilution	Incubation
β2‐AR	ab182136, Abcam, USA	Monoclonal rabbit	1:250/1:1000	Overnight 4°C
Ki‐67	9027, Cell Signaling, USA	Monoclonal rabbit	1:400	Overnight 4°C
Shh	ab53281, Abcam, USA	Monoclonal rabbit	1:400/1:1000	Overnight 4°C
Gli1	bs‐1206R, Bioss, China	Polyclonal rabbit	1:400	Overnight 4°C
	ab49314, Abcam, USA	Polyclonal rabbit	1:1000
Ptch1	ab53715, Abcam, USA	polyclonal rabbit	1:250/1:1000	Overnight 4°C
Cyclin D1	2978, Cell Signaling, USA	Monoclonal rabbit	1:1000	Overnight 4°C
c‐Myc	5605, Cell Signaling, USA	Monoclonal rabbit	1:1000	Overnight 4°C
VEGFA	ab1316, Abcam, USA	Monoclonal mouse	1:1000	Overnight 4°C
GAPDH	97166, Cell Signaling, USA	Monoclonal mouse	1:5000	Overnight 4°C
β‐tubulin	100109‐MM05T, Sino Biological, China	Monoclonal mouse	1:5000	Overnight 4°C

Abbreviations: β2‐AR, β2‐adrenergic receptor; GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; Shh, Sonic Hedgehog; VEGFA, vascular endothelial growth factor.