Table 1.
Tested single amino acid substitutions in the CENH3 histone fold domain that lead to haploid induction at a frequency of 5% or higher
| Codon change | AA change | % Seed death | # Haploids | # Viable progeny | % Haploids |
|---|---|---|---|---|---|
| GGA → GAG | G83E | 36 | 20 | 164 | 12.2 |
| GCT → ACT | A86T | 64 | 4 | 43 | 9.3 |
| GAG → AAG | E89K | 75 | 15 | 34 | 44.1 |
| CGT → TGT | R124C | 42 | 5 | 53 | 9.4 |
| GCG → ACG | A136T | 34 | 5 | 21 | 23.8 |
| TCA → TTA | S148T | 31 | 12 | 155 | 7.7 |
| GCA → ACA | A155T | 48 | 23 | 168 | 13.7 |
| CGT → CAT | R157H | 67 | 6 | 34 | 17.6 |
| ACT → ATT | T159I | 62 | 19 | 97 | 19.6 |
| GGA → AGA | G172R | 41 | 4 | 64 | 6.3 |
| GGA → AGA | G173R | 55 | 9 | 96 | 9.4 |
Plants carrying the mutant allele as a transgene and null for the endogenous gene were pollinated by a CENH3 wild‐type Ler gl1‐1 pollen donor. Haploids were scored using the glabrous phenotype, and % is among viable offspring. Mutant G83E was previously published (Kuppu et al., 2015).