Table 2.

Dilution Factors Antibodies for Support Protocol 2

Antibody mix	Components	Dilution	Volume (µl)
Ectoderm	anti‐FAPB7	1:100	1
	anti‐PAX6	1:200	0.5
	anti‐Nestin	1:200	0.5
	4% NSS		98
Endoderm	anti‐FOXA2	1:500	0.2
	anti‐EOMES	1:100	1
	anti‐GATA4	1:200	0.5
	4% NSS		98.3
Mesoderm	anti‐Vimentin	1:400	0.25
	anti‐CDX2	1:500	0.2
	anti‐Brachyury	1:200	0.5
	4% NSS		99