Figure 4.

Effect of treatment on protein phosphorylation or protein cleavage. Cells were treated with 0.1% dimethyl sulfoxide (DMSO) as control, 10 µM erlotinib, 5 µM crizotinib, or a combination of both for 24 hr. Phosphorylation/protein cleavage was assessed by Pathscan assay. Relative fluorescent units were used and quantified with the Odyssey imaging system. (a) Pathscan assay HCC827; (b) Pathscan assay HCC827GR5. Control: 0.1% DMSO, E: 10 µM erlotinib, C: 5 µM crizotinib, Combo: 10 µM erlotinib + 5 µM crizotinib