Table 1.
Cytotoxicity and anti‐influenza virus activity in MDCK[a] cell cultures.
|
Compound |
Cytotoxicity [μM] |
Antiviral EC50 [d] [μM] |
||||||
|---|---|---|---|---|---|---|---|---|
|
|
|
|
A/H1N1 |
A/H3N2 |
Influenza B |
|||
|
|
MCC[b] |
CC50 [c] |
CPE |
MTS |
CPE |
MTS |
CPE |
MTS |
|
12 |
42 |
4 |
>100 |
>100 |
>100* |
>100* |
>100 |
>100 |
|
13 |
0.8 |
0.8 |
>100 |
>100 |
>100* |
>100* |
>100 |
>100 |
|
15 |
100 |
>100 |
7.7 |
7.2 |
2.3 |
1.9 |
8.9 |
11 |
|
16 |
100 |
97 |
5.6 |
1.6 |
2.3* |
2.5* |
5.6 |
6.5 |
|
17 |
100 |
44 |
6.8 |
8.6 |
1.2 |
1.6 |
4.0 |
3.4 |
|
18 |
– |
4.2 |
– |
>100 |
– |
>100* |
– |
>100 |
|
teicoplanin |
>100 |
>100 |
>100 |
>100 |
>100 |
>100 |
>100 |
>100 |
|
14 |
>100 |
>100 |
>100 |
>100 |
>100 |
>100 |
>100 |
>100 |
|
vancomycin⋅HCl |
>100 |
>100 |
>100 |
>100 |
>100 |
>100 |
>100 |
>100 |
|
zanamivir |
>100 |
>100 |
1.5 |
3.1 |
20 |
9.0 |
2 |
1.7 |
[a] Madin Darby canine kidney cells. Virus strains: A/H1 N1: A/Ned/378/05; A/H3 N2: A/HK/7/87* or A/Victoria/361/11; influenza B virus: B/Ned/537/05. [b] Minimum cytotoxic concentration, i. e., minimal compound concentration causing a microscopically detectable alteration in cell morphology. [c] 50 % cytotoxic concentration based on the formazan‐based MTS cell viability assay. [d] 50 % effective concentration, or concentration producing 50 % inhibition of virus‐induced cytopathic effect, as determined by visual CPE scoring (left column), or by measuring cell viability with the MTS assay (right column).