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. Author manuscript; available in PMC: 2020 Oct 7.
Published in final edited form as: J Leukoc Biol. 2020 May 6;107(6):1123–1135. doi: 10.1002/JLB.2MA0320-383RR

FIGURE 5. CXCL12 variants differentially induce β-arrestin 2 recruitment to the two receptors and receptor-dependent β-arrestin 2 activation.

FIGURE 5.

HEK293T cells were transiently co-transfected with Rluc-β-arrestin 2 and CXCR4-YFP (A) or Rluc-β-arrestin 2 and ACKR3-YFP (B) and stimulated with increasing concentrations of the indicated ligands. BRET data were expressed as percent of the maximal response induced by 1 μM of WT CXCL12. Data represent the mean ±SEM of three independent experiments performed in duplicate. (C-D) Intramolecular BRET assay to monitor receptor-dependent β-arrestin 2 activation. Ligand-promoted conformational changes of β-arrestin 2 were monitored by using Rluc8-β-arrestin 2-Ypet in CXCR4 (C) or ACKR3 (D) transfected cells stimulated by the indicated ligands (1 μM). Data represent the mean ±SEM of three independent experiments performed in duplicate.