TABLE 1.

Primers used for reverse‐transcriptase–polymerase chain reaction

Target genes	Primer sequence: forward	Primer sequence: reverse
GAPDH	5′‐ACCCAGAAGACTGTGGATGG‐3′	5′‐TTCTAGACGGCAGGTCAGGT‐3′
COL1A1	5′‐GTCGAGGGCCAAGACGAAG‐3′	5′‐CAGATCACGTCATCGCACAAC‐3′
COL1A2	5′‐AATTGGAGCTGTTGGTAACGC‐3′	5′‐CACCAGTAAGGCCGTTTGC‐3′
COL2A1	5′‐GTGGAGCAGCAAGAGCAA‐3′	5′‐TGTTGGGAGCCAGATTGT‐3′
COL10A1	5′‐ATGCTGCCACAAATACCCTTT‐3′	5′‐GGTAGTGGGCCTTTTATGCCT‐3′
SOX9	5′‐AACGCCGAGCTCAGCAAGA‐3′	5′‐CCGCGGCTGGTACTTGTAATC‐3′
MMP13	5′‐ACTGAGAGGCTCCGAGAAATG‐3′	5′‐GAACCCCGCATCTTGGCTT‐3′
ACAN	5′‐AGGAGACAGAGGGACACGTC‐3′	5′‐TCCACTGGTAGTCTTGGGCAT‐3′
RUNX2	5′‐ACCATGGTGGAGATCATCG‐3′	5′‐CGCCATGACAGTAACCACAG‐3′

Note: Primer sequences for COL1A1, COL1A2, and COL10A1 from Yamashita et al. (2015).

Abbreviations: ACAN, aggrecan; COL10A1, collagen, type X, alpha 1; COL1A1, collagen, type I, alpha 1; COL1A2, collagen, type I, alpha 2; COL2A1, collagen, type II, alpha 1; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; MMP13, matrix metalloproteinase 13; RUNX2, Runt‐related transcription factor 2; SOX9, SRY‐Box 9.