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. 2020 Apr 28;528(16):2654–2678. doi: 10.1002/cne.24924

FIGURE 5.

FIGURE 5

Differential expression of Atp1a1 and Atp1a3 mRNAs across neuronal subtypes in the mouse hippocampus. (a,b) Double fluorescent in situ hybridization for Atp1a1 and Atp1a3 mRNAs in the hippocampus with opposite combinations of detection probes (a, Atp1a1‐DIG and Atp1a3‐FLU; b, Atp1a1‐FLU and Atp1a3‐DIG). The panels show Atp1a1‐expressing cells (a1, a4, b1, and b4), Atp1a3‐expressing cells (a2, a5, b2, and,5), and a merged view (a3, a6, b3, and b6) in coronal sections of CA1 (a1–a3 and b1–b3) and the dentate gyrus (DG; a4–a6 and b4–b6). Scale bar: 100 μm. (c,d) Scatterplot of signal intensities by Atp1a1‐DIG and Atp1a3‐FLU (c1 and 2) or Atp1a1‐FLU and Atp1a3‐DIG (d). Data of c1 and c2 were obtained from different animals. Signal intensities in the pyramidal cell layer (PCL) of CA1 (blue dots), granule cell layer of the DG (orange dots), and the hilus (gray dots). Each dot represents a single cell (n = 20 cells in each region). Large plots and bars show means ± SD. Statistical significance was calculated using one‐way analysis of variance (ANOVA) with post hoc Tukey's test (n = 20 cells). **, p < .01; ***, p < .001