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. 2020 Apr 28;528(16):2654–2678. doi: 10.1002/cne.24924

FIGURE 8.

FIGURE 8

Differential expression of Atp1a1 and Atp1a3 mRNAs across neuronal subtypes in the mouse somatosensory cortex. (a) Double fluorescent in situ hybridization for Atp1a1 and Atp1a3 mRNAs in the somatosensory cortex (a1–a3, Atp1a1‐DIG and Atp1a3‐FLU; a4–a6, Atp1a1‐FLU and Atp1a3‐DIG). The panels show Atp1a1‐expressing cells (a1 and a4), Atp1a3‐expressing cells (a2 and a5), and a merged view (a3 and a6) in a coronal section of the somatosensory cortex. (b) Magnified view of Layer 2/3 (b1–b3) and Layer 5 (b4–b6) in a1–a3. The arrows show Atp1a1(++)/Atp1a3(++) cells. The arrowheads show Atp1a1(−) Atp1a3(++) cells. Scale bar: 100 μm in a1, 50 μm in b1. (c,d) Scatterplot of Atp1a1‐DIG and Atp1a3‐FLU (c) or Atp1a1‐FLU and Atp1a3‐DIG (d) signal intensities in Layer 2/3 (blue dots) and Layer 5 (orange dots) of the somatosensory cortex. Each dot represents a single cell (n = 50 cells in each layer). Large plots and bars show median with interquartile. Data of c1 and c2 were obtained from different animals. Statistical significance was calculated using Mann–Whitney U test (n = 50 cells). ***, p < .001