FIGURE 9.

Atp1a3‐expressing cells in the mouse superficial somatosensory cortex and deep neocortex. Double fluorescent in situ hybridization for Atp1a3 and Vlgut1 or Gad65/67 mRNAs in the somatosensory cortex. The Atp1a3 (+ and ++) cells in the superficial somatosensory cortex were primarily inhibitory, whereas those in the deep neocortex were both excitatory and inhibitory. (a) Low‐magnification views of Atp1a3 (a1 and a4) and Vglut1 (a2) or Gad65/67 (a5) labeling and a merged view (a3 and a6: magenta, Atp1a3; green, Vglut1 or Gad65/67). (b) High‐magnification views of Layer 2/3 (b1–b6) and Layer 5 (b7–b12) in (a). The arrows show Atp1a3(+) cells positive for the corresponding marker. The arrowheads show Atp1a3(+) cells negative for the corresponding marker. Scale bar: 100 μm in a1 and a4, 50 μm in b1