FIGURE 17.

High levels of Atp1b1 expression and low levels of Atp1b2 expression by PV neurons in the DG and hilus in the mouse brain. (a) Double fluorescent labeling of Atp1b isoform 1 or 2 mRNA (a1 and a4, respectively) and PV protein (a2 and a5), and merged views (a3 and a6: magenta, Atp1a; green, PV) in the DG. Atp1b1 (a1–a3) and Atp1b2 (a4–a6). The arrows show PV(+) and Atp1b1(++) cells. The arrowheads show PV(+) cells. Scale bars: 100 μm. (b) Signal intensities of Atp1b1 and Atp1b2 in a single PV(+) or PV(−) cell. Data represent means ± SD and each dot represents data from a single cell (n = 10 cells). Data of b1 and b2 were obtained from different animals. Statistical significance was calculated using unpaired t test (n = 10 cells). ***, p < .001