Table 3.

Elution program for analytical HIC.

t [min]	Flow rate [mL min−1]	Eluent A [%]	Eluent B [%]	Eluent C [%]
0–10	1	100	–	–
10–15	1	90	10	–
15–24	1	60	40	–
24–38	1	15	85	–
38–45	1	–	100	–
45–52	0.5	–	–	100
52–70	0.5	100	–	–