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HPTLC analysis of the formation of sialyltransfer product α2,3‐SiaLac and hydrolysis side product Neu5Ac by using ▪: native 2,3SiaTpph and variants ▪: A151D, ▪: L387A, ▪: S359T/S360T, ▪: A151D/L387A. Data from duplicate experiments (error bars depicted as standard error of the mean (SEM)) were averaged and corrected with a scaling factor for absolute percentages determined by NMR analysis of endpoint product [%] and side product [%] after 22 h.
