TABLE 1.
Electrophysiological voltage‐clamp measurements determining functional Kv1.3, Kir2.1, P2X4, and P2X7 expression in cultured neonatal mouse microglia
| Cultures | Stimulus | Kv1.3 | Kir2.1 | P2X4 | P2X7 | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Capacitance (pF) | Current (pA) | Density (pA/pF) | Current (pA) | Density (pA/pF) | Current (pA) | Density (pA/pF) | Current (pA) | Density (pA/pF) | ||
| Mixed gender | Undifferentiated | 13.44 ± 5.16 (n = 97) | 32.15 ± 50.50 (n = 97) | 2.68 ± 4.11 (n = 97) | 267.98 ± 368.66 (n = 97) | 17.26 ± 18.17 (n = 97) | 51.67 ± 39.42 (n = 37) | 3.71 ± 2.90 (n = 37) | 1.05 ± 3.15 (n = 19) | 0.09 ± 0.31 (n = 19) |
| IL‐4 | 15.88 ± 4.23 (n = 35) | 30.23 ± 32.22 (n = 30) | 2.09 ± 2.44 (n = 30) | 282.59 ± 233.39 (n = 35) |
9.99 ± 6.46 (n = 35) *p < 5 × 10−3 |
38.66 ± 45.82 (n = 18) |
2.65 ± 3.27 (n = 18) *p < 5 × 10−2 |
3.33 ± 6.51 (n = 12) | 0.25 ± 0.49 (n = 12) | |
| LPS |
18.86 ± 5.99 (n = 116) *p < 5 × 10−11 # p < 1 × 10−2 |
227.13 ± 253.73 (n = 114) *p < 1 × 10−12 # p < 1 × 10−12 |
10.16 ± 10.16 (n = 114) *p < 1 × 10−10 # p < 1 × 10−11 |
65.28 ± 59.31 (n = 114) *p < 1 × 10−6 # p < 5 × 10−6 |
3.55 ± 3.27 (n = 114) *p < 1 × 10−10 |
11.22 ± 17.72 (n = 72) *p < 5 × 10−3 # p < 5 × 10−2 |
0.63 ± 0.99 (n = 72) *p < 5 × 10−3 # p < 5 × 10−2 |
3.44 ± 6.74 (n = 38) | 0.14 ± 0.29 (n = 38) | |
| Female | Undifferentiated | 8.93 ± 2.88 (n = 28) | 17.50 ± 42.21 (n = 28) | 1.95 ± 4.08 (n = 28) | 196.42 ± 151.51 (n = 28) | 21.85 ± 14.06 (n = 28) | 35.78 ± 34.69 (n = 21) | 4.50 ± 4.04 (n = 21) | n.d. | n.d. |
| IL‐4 | 10.64 ± 3.36 (n = 18) | 2.77 ± 11.78 (n = 18) | 0.18 ± 0.78 (n = 18) | 150.55 ± 196.00 (n = 18) |
12.78 ± 15.43 (n = 18) *p < 5 × 10−2 |
50.41 ± 51.41 (n = 12) | 5.13 ± 4.08 (n = 12) | n.d. | n.d. | |
| LPS |
17.20 ± 5.28 (n = 39) *p < 5 × 10−11 # p < 1 × 10−6 |
91.32 ± 159.46 (n = 38) *p < 1 × 10−2 # p < 5 × 10−3 |
6.00 ± 10.04 (n = 38) # p < 5 × 10−3 |
63.84 ± 66.47 (n = 39) *p < 5 × 10−4 |
4.15 ± 4.15 (n = 39) *p < 5 × 10−7 # p < 5 × 10−2 |
7.50 ± 13.04 (n = 30) *p < 5 × 10−3 # p < 5 × 10−2 |
0.54 ± 0.86 (n = 30) *p < 5 × 10−3 # p < 5 × 10−3 |
n.d. | n.d. | |
| Male | Undifferentiated | 8.76 ± 3.05 (n = 33) | 8.75 ± 19.46 (n = 32) | 1.18 ± 2.66 (n = 32) | 232.15 ± 147.52 (n = 33) | 27.09 ± 18.37 (n = 33) | 75.00 ± 66.80 (n = 17) | 8.28 ± 5.22 (n = 17) | n.d. | n.d. |
| IL‐4 | 7.51 ± 2.62 (n = 18) | 12.22 ± 22.21 (n = 18) | 1.23 ± 2.19 (n = 18) |
127.22 ± 113.64 (n = 18) *p < 5 × 10−2 |
17.08 ± 15.81 (n = 18) | 58.57 ± 26.56 (n = 14) | 8.63 ± 5.14 (n = 14) | n.d. | n.d. | |
| LPS |
14.69 ± 4.99 (n = 46) *p < 5 × 10−8 # p < 5 × 10−9 |
118.64 ± 165.80 (n = 44) *p < 1 × 10−4 # p < 5 × 10−4 |
8.45 ± 10.91 (n = 44) *p < 1 × 10−4 # p < 5 × 10−4 |
100.88 ± 68.68 (n = 44) *p < 5 × 10−5 |
7.38 ± 5.22 (n = 44) *p < 1 × 10−6 # p < 5 × 10−2 |
12.93 ± 36.29 (n = 29) *p < 5 × 10−3 # p < 5 × 10−4 |
0.90 ± 2.23 (n = 29) *p < 5 × 10−5 # p < 1 × 10−4 |
n.d. | n.d. | |
Note: n, number of individual cells combined from three or more separate microglia cultures (N). All data represented as mean ± SD; statistical significance among different stimulation conditions (undifferentiated, IL‐4‐treated, and LPS‐treated cells) within or between groups (mixed‐gender, female, or male microglia) determined by one‐way ANOVA with post hoc Tukey–Kramer test (alpha = 0.05). Statistical significance defined as p < 5 × 10−2 for 1). * signifies direct comparison against undifferentiated cells or 2); # signifies direct comparison against IL‐4 differentiated microglia; n.d., data not determined.
Abbreviations: ANOVA, analysis of variance; IL‐4, interleukin‐4; LPS, lipopolysaccharides.