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. 2020 Jun 11;68(11):2377–2394. doi: 10.1002/glia.23847

TABLE 1.

Electrophysiological voltage‐clamp measurements determining functional Kv1.3, Kir2.1, P2X4, and P2X7 expression in cultured neonatal mouse microglia

Cultures Stimulus Kv1.3 Kir2.1 P2X4 P2X7
Capacitance (pF) Current (pA) Density (pA/pF) Current (pA) Density (pA/pF) Current (pA) Density (pA/pF) Current (pA) Density (pA/pF)
Mixed gender Undifferentiated 13.44 ± 5.16 (n = 97) 32.15 ± 50.50 (n = 97) 2.68 ± 4.11 (n = 97) 267.98 ± 368.66 (n = 97) 17.26 ± 18.17 (n = 97) 51.67 ± 39.42 (n = 37) 3.71 ± 2.90 (n = 37) 1.05 ± 3.15 (n = 19) 0.09 ± 0.31 (n = 19)
IL‐4 15.88 ± 4.23 (n = 35) 30.23 ± 32.22 (n = 30) 2.09 ± 2.44 (n = 30) 282.59 ± 233.39 (n = 35)

9.99 ± 6.46 (n = 35)

*p < 5 × 10−3

38.66 ± 45.82 (n = 18)

2.65 ± 3.27 (n = 18)

*p < 5 × 10−2

3.33 ± 6.51 (n = 12) 0.25 ± 0.49 (n = 12)
LPS

18.86 ± 5.99

(n = 116)

*p < 5 × 10−11

# p < 1 × 10−2

227.13 ± 253.73

(n = 114)

*p < 1 × 10−12

# p < 1 × 10−12

10.16 ± 10.16

(n = 114)

*p < 1 × 10−10

# p < 1 × 10−11

65.28 ± 59.31

(n = 114)

*p < 1 × 10−6

# p < 5 × 10−6

3.55 ± 3.27 (n = 114)

*p < 1 × 10−10

11.22 ± 17.72 (n = 72)

*p < 5 × 10−3

# p < 5 × 10−2

0.63 ± 0.99 (n = 72)

*p < 5 × 10−3

# p < 5 × 10−2

3.44 ± 6.74 (n = 38) 0.14 ± 0.29 (n = 38)
Female Undifferentiated 8.93 ± 2.88 (n = 28) 17.50 ± 42.21 (n = 28) 1.95 ± 4.08 (n = 28) 196.42 ± 151.51 (n = 28) 21.85 ± 14.06 (n = 28) 35.78 ± 34.69 (n = 21) 4.50 ± 4.04 (n = 21) n.d. n.d.
IL‐4 10.64 ± 3.36 (n = 18) 2.77 ± 11.78 (n = 18) 0.18 ± 0.78 (n = 18) 150.55 ± 196.00 (n = 18)

12.78 ± 15.43 (n = 18)

*p < 5 × 10−2

50.41 ± 51.41 (n = 12) 5.13 ± 4.08 (n = 12) n.d. n.d.
LPS

17.20 ± 5.28

(n = 39)

*p < 5 × 10−11

# p < 1 × 10−6

91.32 ± 159.46 (n = 38) *p < 1 × 10−2 # p < 5 × 10−3

6.00 ± 10.04 (n = 38)

# p < 5 × 10−3

63.84 ± 66.47 (n = 39)

*p < 5 × 10−4

4.15 ± 4.15 (n = 39)

*p < 5 × 10−7

# p < 5 × 10−2

7.50 ± 13.04 (n = 30)

*p < 5 × 10−3

# p < 5 × 10−2

0.54 ± 0.86 (n = 30)

*p < 5 × 10−3

# p < 5 × 10−3

n.d. n.d.
Male Undifferentiated 8.76 ± 3.05 (n = 33) 8.75 ± 19.46 (n = 32) 1.18 ± 2.66 (n = 32) 232.15 ± 147.52 (n = 33) 27.09 ± 18.37 (n = 33) 75.00 ± 66.80 (n = 17) 8.28 ± 5.22 (n = 17) n.d. n.d.
IL‐4 7.51 ± 2.62 (n = 18) 12.22 ± 22.21 (n = 18) 1.23 ± 2.19 (n = 18)

127.22 ± 113.64 (n = 18)

*p < 5 × 10−2

17.08 ± 15.81 (n = 18) 58.57 ± 26.56 (n = 14) 8.63 ± 5.14 (n = 14) n.d. n.d.
LPS

14.69 ± 4.99

(n = 46)

*p < 5 × 10−8

# p < 5 × 10−9

118.64 ± 165.80

(n = 44)

*p < 1 × 10−4

# p < 5 × 10−4

8.45 ± 10.91 (n = 44)

*p < 1 × 10−4

# p < 5 × 10−4

100.88 ± 68.68 (n = 44)

*p < 5 × 10−5

7.38 ± 5.22 (n = 44)

*p < 1 × 10−6

# p < 5 × 10−2

12.93 ± 36.29 (n = 29)

*p < 5 × 10−3

# p < 5 × 10−4

0.90 ± 2.23 (n = 29)

*p < 5 × 10−5

# p < 1 × 10−4

n.d. n.d.

Note: n, number of individual cells combined from three or more separate microglia cultures (N). All data represented as mean ± SD; statistical significance among different stimulation conditions (undifferentiated, IL‐4‐treated, and LPS‐treated cells) within or between groups (mixed‐gender, female, or male microglia) determined by one‐way ANOVA with post hoc Tukey–Kramer test (alpha = 0.05). Statistical significance defined as p < 5 × 10−2 for 1). * signifies direct comparison against undifferentiated cells or 2); # signifies direct comparison against IL‐4 differentiated microglia; n.d., data not determined.

Abbreviations: ANOVA, analysis of variance; IL‐4, interleukin‐4; LPS, lipopolysaccharides.