TABLE 3.
Effects of Kv1.3 inhibition on microglial RMP and P2X4‐mediated membrane potential changes
| − ShK‐223 | + ShK‐223 | |||||
|---|---|---|---|---|---|---|
| Treatments | RMP (mV) | AMP (mV) | AID (mV) | RMP (mV) | AMP (mV) | AID (mV) |
| Undifferentiated | −88.05 ± 6.08 (n = 14) |
−16.30 ± 9.11 (n = 14) *p < 5 × 10−13 |
71.74 ± 9.11 (n = 14) | −85.47 ± 8.05 (n = 14) |
−12.36 ± 12.29 (n = 14) *p < 5 × 10−12 |
73.11 ± 10.56 (n = 14) |
| LPS | −73.37 ± 9.34 (n = 8) |
−47.93 ± 5.52 (n = 8) *p < 5 × 10−4 |
25.44 ± 9.35 (n = 8) |
−45.39 ± 2.93 (n = 8) # p < 5 × 10−12 |
−25.36 ± 8.16 (n = 8) *p < 5 × 10−4 # p < 5 × 10−5 |
20.04 ± 7.54 (n = 8) |
Note: Effects of Kv1.3 inhibition by 100 nM ShK‐223 on RMP, AMP by 100 μM ATP, and AID. “n,” number of individual cells from three or more separate microglia cultures. All data represented as mean ± SD. Paired t test was used to determine statistical significance between before and after ATP (*) or before and after ShK‐223 (#). Statistical significance defined as p < 5 × 10−2.
Abbreviations: AID, ATP‐induced depolarization; AMP, ATP‐induced membrane potential; LPS, lipopolysaccharides; RMP, resting membrane potential.