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. Author manuscript; available in PMC: 2020 Oct 7.
Published in final edited form as: Nat Microbiol. 2020 Mar 9;5(6):813–820. doi: 10.1038/s41564-020-0687-z

Extended Data Fig. 7: E. coli PBP2 variants co-purify with E. coli RodA.

Extended Data Fig. 7:

PBP2 variants were co-expressed with FLAG-tagged RodA and purified using anti-FLAG affinity resin. SDS-PAGE gel showing the elution from the anti-FLAG affinity resin demonstrates that PBP2 remains associated with RodA throughout the purification. Low (L) and high (H) correspond to approximately 2 μg and 4 μg as measured by A280. Results are derived from one experiment.