Skip to main content
. Author manuscript; available in PMC: 2021 Aug 6.
Published in final edited form as: Cell. 2020 Jul 16;182(3):578–593.e19. doi: 10.1016/j.cell.2020.06.031

Figure 1. Sympathectomy delays anagen entry whereas elevation of sympathetic tone drives anagen entry.

Figure 1.

(A) Immunofluorescent staining for tyrosine hydroxylase (TH) in control and sympathectomized (6-OHDA) skin. (B) Immuno-colocalization of EdU, CD34, and P-Cadherin (PCAD) in control and sympathectomized P25 skin. (C) Hematoxylin & Eosin (H&E) staining of control and sympathectomized skin. Graph: hair cycle distribution at P30 (n = 4 – 5 mice per condition, 20 hair follicles (HF) per mouse). (D) H&E staining of control and sympathectomized TH-CreER; Rosa-lsl-attenuated DTA (TH-CreER; DTA) skin. Graph: hair cycle distribution at P31 - P34 (n = 4 – 5 mice per condition, 10 HF per mouse). (E) Topical application of isoproterenol at the 2nd telogen results in precocious anagen entry (n = 10 mice per condition). Graph: back skin hair regrowth (%). Unless otherwise specified, all scale bars = 50 μm. Data are mean ± SEM. *: p<0.05; **: p<0.01; ***: p <0.001. See also Figure S1.