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. Author manuscript; available in PMC: 2021 May 15.
Published in final edited form as: Physiol Behav. 2020 Feb 18;219:112847. doi: 10.1016/j.physbeh.2020.112847

Figure 4. Effects of CVS on the number of AVPV/PeN CRFR1-GFP, c-Fos/CRFR1, and pCREB/CRFR1 cells.

Figure 4.

(A) CVS females showed a significant increase in CRFR1-ir compared to all other treatment groups. (B) Representative images of CVS and NS female and male AVPV/PeN CRFR1-GFP. (C) c-Fos/CRFR1-GFP co-expression was significantly higher in all female AVPV/PeN than male, regardless of treatment condition. (D) Representative images of CVS and NS female and male c-Fos/CRFR1-GFP showing a female-specific reduction in c-Fos/CRFR1-GFP cells. (E) Females, regardless of treatment, had increased pCREB/CRFR1-GFP in the AVPV/PeN compared to males, and CVS females specifically had higher pCREB/CRFR1-GFP than any other groups. (F) Representative images of NS and CVS female and male pCREB/CRFR1-GFP in the AVPV/PeN showing female specific increased pCREB. 3V, third ventricle; NS, non-stressed; CVS, chronic variable stress. * Indicates a main effect of sex, (F>M; p < 0.05). + indicates a significant increase in CVS compared to NS females (p < 0.01).