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. Author manuscript; available in PMC: 2021 May 15.
Published in final edited form as: Physiol Behav. 2020 Feb 18;219:112847. doi: 10.1016/j.physbeh.2020.112847

Figure 7. Effects of CVS on CRFR1-GFP cell number and co-localization with c-Fos and pCREB in various brain regions.

Figure 7.

CRFR1-GFP, CRFR1/c-Fos, and CRFR1/pCREB co-labeled cells were quantified within the anteroventral portion of the bed nucleus of the stria terminalis (BSTav; A-C), dorsolateral portion of the bed nucleus of the stria terminalis (BSTdl; D-F), central amygdala (CeA; G-I), ventral basolateral amygdala (BLAv; J-L), and arcuate nucleus (ARC; M-O). CVS selectively reduced c-Fos/CRFR1-GFP co-labeled cells in female but not male BSTav (B). In the BLAv, the number of CRFR1/c-Fos co-labeled cells was significantly reduced in both sexes (K). There was also a trend toward a reduction in c-Fos/CRFR1 co-labeled cells in CVS mice within the arcuate nucleus (p=0.056; N), with CVS animals regardless of sex, trending toward reduced co-expression. No significant differences were found within the BSTdl or CeA. Data are reported as mean cells/mm2 ± SEM. + Indicates main effect of stress on outcome, p≤0.05. * Indicates an effect of sex on outcome, p≤0.05. & indicates a trend toward main effect of treatment, p = 0.056. NS, non-stressed; CVS, chronic variable stress.