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. Author manuscript; available in PMC: 2021 Aug 5.
Published in final edited form as: Neuron. 2020 Jun 22;107(3):454–469.e6. doi: 10.1016/j.neuron.2020.05.005

Figure 1. Ribosome Tethering Enables Ultrasensitive Visualization of GFP Reporters.

Figure 1.

(A) Synthetic proteins tagged with ribosomal unit L10a become tethered to ribosomes and are enriched in the soma.

(B) The Nano-L10 transgenic mouse expresses a GFP-binding nanobody fused to ribosomal protein L10 in all neurons. When crossed to a second transgenic line expressing GFP from a cell-type-specific promoter, the GFP is captured by the nanobody, anchoring it in the soma.

(C–H) Images from the brains and peripheral ganglia of GFP reporter mice, either alone (bottom row) or after crossing to the Nano-L10 mouse (top row). All of the images show direct GFP fluorescence, except for (D), which is immunostained for GFP. Panels show Agtr1a-GFP (C), GAD67-GFP (D), GCG-GFP (E), TRPV1-GFP

(F), TRPM8-GFP (G), and TrkB-tauGFP (H). Scale bar sizes are indicated in each panel. See also Figure S1.