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. Author manuscript; available in PMC: 2021 Aug 5.
Published in final edited form as: Neuron. 2020 Jun 22;107(3):454–469.e6. doi: 10.1016/j.neuron.2020.05.005

Figure 3. Ribo-tagging Does Not Change the Kinetics of GCaMP in Response to Evoked Neural Activity.

Figure 3.

(A) Schematic describing the measurement of fluorescence in response to electrically evoked neural activity in DG. Grayscale heatmap shows the brightness of DG neurons expressing ribo-GCaMP6m before and during pulsed field stimulation.

(B) Response of ribo-GCaMP6m and regular GCaMP6m to different numbers of electrical field pulses (FPs). When calculating ΔF/F, the average fluorescence during the 300-ms time window before the start of the first FP was used as F0 (the denominator).

(C–E) Peak signal (C; p 0.71, 0.66, 0.65, 0.60, 0.67, < 0.01, 0.67, and 0.16), decay time constant (D; p 0.95, 0.27, 0.96, 0.95, 0.65, 0.87, < 0.01, and < 0.0001), and rise time constant (E; p 0.84, 0.84, 0.25, 0.84, 0.78, 0.43, < 0.0001, and 0.06) for ribo-GCaMP6m (green, n = 304 from 4 brain slices of 2 mice) and GCaMP6m (black, n = 137 from 4 brain slices of 2 mice) in response to the increasing numbers of FPs.