KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Chicken anti-GFP	Abcam	Abcam Cat# ab13970, RRID:AB_300798
Rabbit anti-Iba1	Wako	Wako Cat# 019–19741, RRID:AB_839504
Goat anti-chicken Alexa 488	Invitrogen	Thermo Fisher Scientific Cat# A-11039, RRID:AB_2534096
Goat anti-rabbit Alexa 568	Invitrogen	Thermo Fisher Scientific Cat# A-11036, RRID:AB_10563566
Bacterial and Virus Strains
AAV5- EF1a-DIO-hChR2(H134R)-mCherry-WPRE-HGHpA	Gift from Karl Deisseroth; unpublished	Karl Deisseroth Addgene plasmid #20297
Plasmid: pAAV-hSyn-GCaMP6m-RPL10a-WPRE-HGH	This paper; AAV8 produced by Stanford Vector Core	N/A
Plasmid: pAAV-hSyn-GCaMP6f-RPL10a-WPRE-HGH	This aper; AAV8 produced by Stanford Vector Core	N/A
Plasmid: pAAV-hSyn-GCaMP6s-RPL10a-WPRE-HGH	This paper; AAV8 produced by Stanford Vector Core	N/A
Plasmid: pAAV-hSyn-GCaMP6m-WPRE-HGH (control vector)	This paper; AAV8 produced by Stanford Vector Core	N/A
Plasmid: pAAV-hSyn-DIO-GCaMP6m-RPL10a-WPRE-HGH	This paper; AAV8 produced by Stanford Vector Core	N/A
Plasmid: pAAV-hSyn-DIO-GCaMP6f-RPL10a-WPRE-HGH	This paper; AAV5 produced by Stanford Vector Core	N/A
Plasmid: pAAV-hSyn-DIO-GCaMP6s-RPL10a-WPRE-HGH	This paper	N/A
Chemicals, Peptides, and Recombinant Proteins
CNQX	Tocris	Cat# 0190
(R)-CPP	Tocris	Cat# 00247
Gabazine	Tocris	Cat# 01262
(S)-MCPG	Tocris	Cat# 00337
Tetramisole Hydrochloride	Sigma-Aldrich	CAS# 5086-74-8
DAPI fluoromount-G	Southern Biotech	Cat# 0100–20
Experimental Models: Organisms/Strains
M. musculus: C57BL/6J (WT)	JAX	JAX: 000664, RRID: IMSR_JAX:000664
M. musculus: Tg(Sim1-cre)1Lowl/J (SIM1-Cre)	JAX	JAX: 006395, RRID: IMSR_JAX:006395
M. musculus: Tg(Agtr1a-EGFP)NZ44Gsat (Agtr1a-EGFP)	GENSAT	MGI:4846843
GCG-GFP	Gift from Hayashi Yoshitaka; Hayashi et al., 2009	N/A
GAD67-GFP	Gift from Allan Basbaum, Tamamaki et al., 2003	N/A
TrkB-tauGFP	Gift from David Ginty; Lietal., 2011	N/A
TRPV1-GFP-DTR	Gift from Mark Hoon; Pogorzala et al., 2013	N/A
TRPM8-GFP-DTR	Gift from Mark Hoon; Pogorzala et al., 2013	N/A
Nano-L10	Ekstrand et al., 2014	N/A
M. musculus: B6;129S4-Ntrk1tm1(cre)Lfr/Mmucd (Ntrk1-Cre)	MMRRC	RRID: MMRRC_015500-UCD
C. elegans: Strain N2	Caenorhabditis Genetics Center	WB Strain: 00000001
C. elegans: Strain KG1180: lite-1(ce314) X.	Caenorhabditis Genetics Center	WB Strain: 00023485
C. elegans: Strain JAZ279: jlgEx289[Psra6::GCaMP6m:: rpl-1a;Punc-122::dsRed2]	This study	N/A
C. elegans: Strain JAZ312: jlgEx301[Psra6::GCaMP6m; Punc-122::GFP]	This study	N/A
C. elegans: Strain JAZ275: jlgEx285[Pntc-1::GCaMP6m:: rpl-1a;Punc-122::dsRed2]	This study	N/A
C. elegans: Strain JAZ276: lite-1(ce314) X; jlgEx286[Ptag-168::GCaMP6m::rpl1a;Punc-122::dsRed2];otIs355 [Prab3::2xNLS::tagRFP]	This study	N/A
C. elegans: Strain JAZ313: lite-1(ce314) X; jlgEx302[Prab-3::GCaMP6m::rpl1a;Pelt-2::2xNLS::GFP];otIs355 [Prab3::2xNLS::tagRFP]	This study	N/A
Software and Algorithms
FIJI	Schindelin et al., 2012	https://imagej.net/Fiji/Downloads
Psychtoolbox3	Kleiner et al., 2007	https://github.com/Psychtoolbox-3/Psychtoolbox-3
NormCorre	Pnevmatikakis and Giovannucci, 2017	https://github.com/flatironinstitute/NoRMCorre
Suite2P	Pachitariu et al., 2017	https://github.com/cortex-lab/Suite2P
Capacitive Sensing Library	Arduino Playground	https://playground.arduino.cc/Main/CapacitiveSensor/
CNMF_E	Zhou et al., 2018	https://github.com/zhoupc/CNMF_E
PCA/ICA	Mukamel et al., 2009	https://github.com/mukamel-lab/CellSort
NeuroSeg	Guan et al., 2018	https://github.com/baidatong/NeuroSeg
PRISM 7	GraphPad	https://www.graphpad.com RRID:SCR_005375
Mosaic	Inscopix	https://support.inscopix.com/mosaic-workflow RRID:SCR_017408
MATLAB R2019a	MathWorks	https://www.mathworks.com
Python (Custom Code)	This study	https://github.com/focolab/ribo-gcamp/
Other
Ø500 μm gradient index (GRIN) lens (6.1 mm length)	Inscopix	N/A