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. Author manuscript; available in PMC: 2021 Sep 22.
Published in final edited form as: Vaccine. 2020 Aug 20;38(41):6357–6362. doi: 10.1016/j.vaccine.2020.08.023

Figure 4. Airway mucus production is elevated in RSV-experienced young mice vaccinated with alum-adjuvanted RSV PreF.

Figure 4.

Mice were challenged, immunized, and re-challenged with RSV as described in Figure 1. At 4 or 8dpi, lungs were formalin-filled, paraffin-embedded and sectioned for staining with H&E (Aa-f). Lungs were scored, averaged, and inflammatory score graphed. Data is represented as mean ± SEM (n=3) (B). Inflammatory score trends from 4 to 8dpi are shown in (C). PAS staining was also performed at 4dpi (De-f). To quantify the extent of PAS staining, lungs were scored as described in the methods. Scores were averaged and the total percentage of PAS+ airways were graphed (E). PAS staining quantification trends from 4 to 8dpi are shown in (F). Statistical significance between vaccination groups was calculated in using one-way ANOVA with Tukey’s multiple comparison post-test (B and E) or 2-way ANOVA with Sidak’s multiple comparison post-test (C and F) between all groups. *p<0.05.