Figure 6.

Malformations due to Au NP toxicity in developing zebra fish embryos. Zebra fish at 24 hpf (A1–A12), 48 hpf (B1–B12), and 72 hpf (C1–C12) and (D1–D12) were exposed to, E3 (A12, B12, C12, D12), GtAu(A1–A5, B1–B5, C1–C5, D1–D5), OpAu (A6–A10, B6–B10, C6–C10, D6–D10) and Cd²⁺ 100 ppm (A11, B11, C11, D11). Embryos were scored for number of somites including tail detachment, pericardial edema, normal yolk body size and yolk extension. Embryos exposed to OpAu NPs (C6, C7, C8) were less pigmented than untreated embryos. Embryos exposed to 208.32 ppm, 166.67 ppm OpAu NPs had an increased flattened yolk sac with increased width (C6, C7); lateral view. Embryo exposed to Cd²⁺ 100 ppm was unfertilized. Scale bar, 200 µm.