Figure 1.

Effect of Lipofundin on cell viability and lipid droplet formation in human astrocytes. (a) LDH release in human astrocytes. Cells were treated with the indicated Lipofundin concentration for 5 days. Thereafter, LDH was determined in supernatants and cell lysates. LDH was analysed in relation to intracellular LDH content for each condition. Cells cultured without Lipofundin were set as 100%. Boxplots show data of eight independent experiments and all data points are displayed. There were significant differences between the groups (ANOVA, p = 0.0024). * indicates significant difference from the other conditions in a post hoc unpaired t-test. (b,c) Visualization of lipid droplet formation by Oil Red O staining. Human astrocytes were cultured for 7 consecutive days with either 0.05% Lipofundin (b) or 0.005% Lipofundin (c). A subset of cells was cultured for 3 more days without Lipofundin in the culture media. After fixation, samples were stained with Oil Red O.