Table 3. Intake and diet digestibility of Holstein x Gyr crossbred heifers on a rotational grazing system Panicum maximum cv. Mombaça pasture.

Item1	Supplement2			SEM3	P-Value4
	CON	S12CP	S24CP		CON × SUP	S12CP × S24CP	PER	TR × PER
Intake
DM, kg/d	7.36	8.04	8.58	0.307	0.014	0.220	0.091	0.360
Pasture, kg/d	7.36	6.36	6.84	0.307	0.051	0.270	0.064	0.339
NDF, kg/d	4.86	4.41	4.78	0.201	0.285	0.188	0.027	0.306
CP, kg/d	1.28	1.30	1.63	0.053	0.008	0.001	0.327	0.408
CP/DM, kg/kg	0.17	0.16	0.19	0.0004	0.043	0.001	0.001	0.005
DE, Mcal/d	18.12	21.23	22.33	0.839	0.001	0.355	0.050	0.512
DM/BW, g/kg of BW	19.65	20.81	21.54	0.001	0.278	0.646	0.006	0.297
P/BW, g/kg of BW	19.65	16.49	17.23	0.001	0.056	0.641	0.004	0.301
NDF/BW, g/kg of BW	12.99	11.42	12.04	0.0007	0.176	0.553	0.002	0.259
Digestibility
DM, g/kg	566.10	611.20	614.40	0.009	0.001	0.814	0.001	0.875
NDF, g/kg	696.10	698.20	696.60	0.005	0.864	0.844	0.001	0.893
CP, g/kg	677.30	636.70	698.60	0.015	0.608	0.010	0.001	0.472
Microbial Synthesis
CPmic, g/d	532.10	512.40	527.74	36.954	0.794	0.772	0.924	0.284
EMS, g/kg	131.34	109.28	110.89	8.985	0.072	0.900	0.001	0.5632

¹DM = dry matter; NDF = neutral detergent fiber; CP = crude protein; DE = Digestible energy; CP/DM = Crude protein per dry matter; DM/BW = dry matter per body weight; P/BW = pasture per body weight; NDF/BW = neutral detergent fiber per body weight; CPmic = microbial crude protein; EMS = efficiency of microbial crude protein synthesis (g of CPmic/kg of total digestible nutrients intake).

²CON = not supplemented; S12CP: supplemented with concentrate containing 12% CP; S24CP: supplemented with concentrate containing 24% CP.

³Standard error of the mean.

⁴CON × SUP = effect of supplementation; S12CP × S24CP = effect between protein levels in the supplement; PER = effect of period; TR×PER = interaction effect between supplementation and period.