Table 5. Blood parameters of Holstein x Gyr crossbred heifers on a rotational grazing system Panicum maximum cv. Mombaça pasture.

Item	Supplement1			SEM2	P-Value3
	CON	S12CP	S24CP		CON × SUP	S12CP × S24CP	PER	TR × PER
Urea, mg/dL	33.45	31.75	37.08	1.286	0.551	0.010	0.001	0.002
Glucose, mg/dL	72.87	76.75	74.66	1.647	0.180	0.385	0.001	0.031
Albumin, mg/dL	2.86	3.05	3.17	0.066	0.008	0.233	0.358	0.621
Total protein, mg/dL	7.37	7.45	7.37	0.177	0.873	0.782	0.063	0.486
Total Cholesterol, mg/dL	89.62	93.91	78.87	6.656	0.697	0.130	0.941	0.850
Triglycerides, mg/dL	7.66	8.00	8.70	0.694	0.427	0.485	0.002	0.342
Insulin, μUI/mL	2.25	2.58	2.55	0.406	0.534	0.963	0.009	0.846
IGF-I, ng/mL	227.08	231.87	225.75	27.545	0.959	0.877	0.002	0.804

¹CON = not supplemented; S12CP: supplemented with concentrate containing 12% CP; S24CP: supplemented with concentrate containing 24% CP.

²Standard error of the mean.

³CON × SUP = effect of supplementation; S12CP × S24CP = effect between protein levels in the supplement; PER = effect of period; TR×PER = interaction effect between supplementation and period