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. 2020 Oct 7;15(10):e0240169. doi: 10.1371/journal.pone.0240169

Fig 1. Immunophenotyping of thymocytes and splenocytes in the SRG rat.

Fig 1

A-C) CD4+/CD8+ mature T cells in A) wild type control and B) SRG rat thymocytes. C) Quantification of data, n = 3, error ± SD. (Unpaired t-test, p-values: **** < 0.0001). CD4+/CD8+ mature T cells are absent from SRG thymocytes, compared to a wild-type control. The lack of thymus tissue in the SRG rat results in a low recovery of viable thymocytes. D-F) CD45R (B220)+/IgM+ cells in D) wild-type spleen and E) the SRG spleen. F) Quantification of data, n = 3, error ± SD. (Unpaired t-test, p-values: * < 0.05). Compared to B cells in a wild-type spleen, the SRG spleen contains no mature B cells as demonstrated by lack of CD45R (B220)+/IgM+ cells. G-I) NK cells in G) wild-type rat spleen and H) SRG rat spleen. I) Quantification of data, n = 3, error ± SD. (Unpaired t-test, p-values: * < 0.05). NK cells in the SRG rat spleen (H) are similar to or less than the amount of NK cells in the wild-type rat. The Il2rg knockout in the SRG rat results in significantly fewer NK cells than the single Rag2 knockout rat [8]. J) Image of wild-type Sprague Dawley versus SRG thymus. K) Images of wild-type Sprague Dawley and SRG rat spleen. L) Quantitative comparison of wild-type Sprague Dawley versus SRG spleen and thymus at 8 weeks of age. Data represent average of 3 from each strain with SEM (Unpaired t-test, p-values: **** < 0.0001).