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. 2020 Aug 11;111(10):3626–3638. doi: 10.1111/cas.14545

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© 2020 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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A, Flow cytometric immunophenotyping of blood of Foxn1^nu.B6‐Tg(CAG‐EGFP) (left panel) and immunocompetent mice (right panel), showing almost complete absence of CD3⁺ T cells in Foxn1^nu.B6‐Tg(CAG‐EGFP) mouse (light blue arrow). B, Skin transplantation assay indicated, during 3‐mo observation, that there was no significant immune repulsion among individuals, and the length of newborn hair may vary. C, EGFP⁺ skin from Foxn1^nu.B6‐Tg(CAG‐EGFP) mouse was transplanted into EGFP⁻ BALB/c nude mouse, which still emits intense green light after 3 mo. D, U87 cells showed 100% (5/5) tumorigenic rate in Foxn1^nu.B6‐Tg(CAG‐EGFP) mice