TABLE 1.
Emerging methodologies for multiplexed spatial relationship analyses
| Extending spatial capability to multiplexed analysis | Extending multiplex capability to imaging methods |
|---|---|
| Transcriptomics | |
| Spatial mapping of scRNAseq | Multiplexed fluorescence in situ hybridization |
| In situ hybridization of landmark genes 46 | SeqFISH 51 |
| Reporter‐based cell sorting (NICHE‐seq) 47 | MERFISH 52 |
| Spatial transcriptome profiling | |
| Spatial transcriptomics 48 | |
| Slide‐seq | |
| Digital spatial profiling 50 | |
| Proteomics | |
| High parameter cytometry imaging | Multiplex immunofluorescence |
| Imaging mass cytometry 53 | Antibody stripping (Opal) 55 |
| Multiplexed ion beam imaging (MIBI) 54 | Bleaching 56 |
| Oligonucleotide 57 | |
| Multiplex IHC | |
| Multiplex IHC and image cytometry 3 | |