FIGURE 4.

Inhibition of bromo‐ and extraterminal domain (BET) proteins combined with anti‐programmed cell death‐ligand 1 (PD‐L1) Abs effectively suppressed the progression of hepatocellular carcinoma. A, Schematic of the experimental procedure for JQ1, anti‐PD‐L1 Ab, or the combination treatment. Plasmids were transfected into all mice at day 0. JQ1 (50 mg/kg) (or vehicle solution) was i.p. injected at days (D) 14, 16, 18, 20, and 22, and anti‐PD‐L1 Ab (or isotype IgG) was i.p. injected at days 17, 19, and 21. All mice were killed (SAC) 6 weeks after oncogene transfection for phenotypic analysis. B‐E, Tumor loads were evaluated by liver weight (LW) / body weight (BW) ratios (B), spleen weight (SW) / BW ratios (C), maximal diameters (D), and the numbers of nodules (E). Data are shown as mean ± SEM (*P < .05, **P < .01). NS, P ≥ .05