FIGURE 6.

lncARSR upregulates HK1 expression by competitively binding miR‐34a‐5p. A, Bioinformatically predicted paired bases of miR‐34a‐5p in lncARSR and hexokinase 1 (HK1) 3′ untranslated region (3ʹUTR). B, Protein levels of HK1 in Caco‐2 cells transfected with lncARSR or lncARSR + miR‐34a‐5p mimics and HCT‐8 cells transfected with sh‐lncARSR‐1 or sh‐lncARSR‐1 + miR‐34a‐5p inhibitor. C, D, AGO2‐RNA immunoprecipitation (RIP) followed by qPCR analysis to evaluate HK1 levels after lncARSR knockdown or overexpression. E, Schematic images of a construct containing lncARSR combined with the MS2 binding sequence. F, Green fluorescent protein (GFP)‐RIP followed by qPCR analysis to measure miR‐34a‐5p endogenously combined with lncARSR. G, H, Effects of miR‐34a‐5p knockdown or overexpression on luciferase reporter activity with wild‐type and mutant lncARSR. I, Changes in HK1 mRNA after Caco‐2 cells were transfected with lncARSR and HCT‐8 cells were transfected with sh‐lncARSR‐1. J, K, Effects of miR‐34a‐5p knockdown or overexpression on luciferase reporter activity with the wild‐type and mutant HK1 3ʹUTR. L, M, Effects of miR‐34a‐5p knockdown or overexpression on lncARSR‐regulated luciferase reporter activity with wild‐type and mutant HK1 3ʹUTR. N, Pearson correlation analysis of lncARSR and HK1 mRNA expression in tissue specimens from 89 patients with colorectal cancer (CRC). *P < .05, **P < .01, ***P < .001; ns, not significant