FIGURE 9.

lncARSR promotes colorectal cancer (CRC) cell migration, invasion, and glucose metabolism in vitro and enhanced liver metastasis of nude mice in vivo via competitively binding to miR‐34a‐5p. Caco‐2 cells were transfected with control, lncARSR, or lncARSR + miR‐34a‐5p mimics and HCT‐8 cells were transfected with shCtrl, sh‐lncARSR‐1, or sh‐lncARSR‐1 + miR‐34a‐5p inhibitor. A, B, Transwell assays were used to analyze the migratory and invasive abilities of CRC cells. A series of metabolic parameters was measured, including (C) glucose uptake, (D) lactate production, and (E) cellular ATP levels. *P < .05, **P < .01. F, G, Nude mice were separated into 6 groups (n = 5/group) and injected with the above indicated groups cells via the spleen. The number of metastatic colonies in hematoxylin‐eosin (HE) stained images of liver tissue was counted 4 wk postinjection. *P < .05; **P < .01