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. 2020 Aug 13;111(10):3653–3664. doi: 10.1111/cas.14587

FIGURE 2.

FIGURE 2

Changes in the expression of DHCR24 does not affect the adherent growth of the breast cancer cell lines. A, Knockdown of DHCR24 expression in breast cancer cell lines BT474 and AU565 using PLKO.1 lentivirus expressing control shRNA (csh) and 2 different DHCR24 shRNAs (sh11 and sh12). B, Overexpression of DHCR24 in breast cancer cell lines BT474, SUM149PT, and MCF7 using vector only pBabe‐puro retrovirus or pBabe‐puro retrovirus expressing DHCR24. DHCR24 levels in the indicated breast cancer cell lines were verified by western blot analysis using DHCR24 antibody and β‐Actin antibody as a loading control. DHCR24 knockdown (C) or overexpression (D) does not affect the adherent growth of the indicated breast cancer cell lines. Breast cancer cell lines were subjected to colony formation