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. 2020 Aug 31;111(10):3693–3704. doi: 10.1111/cas.14611

FIGURE 5.

FIGURE 5

LINC00973 functioned as ceRNA against miR‐7109 in clear‐cell renal cell carcinoma (ccRCC). (A) Relative expression of miR‐7109 in both 769‐p and Caki‐1 cells transfected with either negative control or LINC00973‐specific shRNA. (B) Relative expression of miR‐7109 in both A704 and A498 cells transfected with either control or LINC00973‐expressing plasmids. (C) RNA‐pulldown results in both A704 and A498 cells with control, wild‐type LINC0973 fragment, or corresponding mutant, respectively. (D) Relative expression of LINC00973 in both A704 and A498 cells transfected with either negative control or miR‐7109 inhibitors. (E) Relative expression of LINC00973 in both 769‐p and Caki‐1 cells transfected with either control or miR‐7109 mimics. (F) RNA‐pulldown results in both 769‐p and Caki‐1 cells with either control or miR‐7109. (G) Reverse correlation analysis between endogenous miR‐7109 and LINC00973 in ccRCC tumor samples (n = 100). (H) Illustration of aligned miR‐7109 with both wild‐type and mutated LINC00973. (I) Luciferase reporter results in 293T cells co–transfected with LINC00973 (wt and mut) and miR‐NC, miR‐7109 mimic, and miR‐7109 inhibitor, respectively