Figure 7.

In vivo antitumor effects of double or triple combination of chemotherapeutic drugs with anti‐PD‐1 antibody therapy. A, C57BL/6 mice were injected subcutaneously (s.c.) with 5.0 × 10⁵ MC38 cells into the right flank. On days 10 and 18, the mice were injected intraperitoneally (i.p.) with CP (50 mg/kg) or 5‐FU (50 mg/kg) and L‐OHP (6 mg/kg). On days 11 and 19, anti‐PD‐1 antibody (200 μg/mouse) was injected i.p. The tumor volume was calculated as follows: (length × width²)/2. The black and red arrows indicate the injection times of chemotherapeutic drugs and antibody, respectively. The number in parentheses represents the ratio of cured mice to total mice. B, Tumor volume data on day 26. The data are presented as means ± SEM. C, D, The body weight data on days 14, 22, and 26 are shown as means ± SEM. * P < 0.05, ** P < 0.01 (ANOVA). CP, cyclophosphamide; 5‐FU, 5‐fluorouracil; L‐OHP, oxaliplatin