FIGURE 3.

Transcriptional coactivator with PDZ binding domain (TAZ) overexpression is associated with radiation resistance in esophageal cancer cells. A, B, Cell surviving fractions of TAZ‐overexpressing cells (A), TAZ‐depleted cells (B), and their respective control cells. C, D, Apoptotic assays of TAZ‐overexpressing cells (C), TAZ‐depleted cells (D), and their respective control cells post‐irradiation (6 Gy). Left panels, representative pictures; right panels, quantifications of annexin‐positive cells. E, F, Photographs of mice (E) and tumors (F) with or without irradiation. G, H, Volume (G) and weight (H) of xenografts with or without irradiation. Each dot represents one sample. I, Inhibition rate of irradiation on xenograft tumors. Inhibition rate = 1 − tumor weight with irradiation / tumor weight without irradiation. Mean ± SD of at least three independent experiments. *P < .05, **P < .01, ***P < .001. EV, empty vector; IR, irradiation; siScr, scramble siRNA; siTAZ1/2, siRNA against TAZ mRNA 1/2; TAZ, plasmid containing TAZ