FIGURE 4.

Transcriptional coactivator with PDZ binding domain (TAZ) promoted the expression of multiple genes involved in nonhomologous end joining (NHEJ). A, B, Dynamic levels of γ‐H2AX in TAZ‐overexpressing TE‐1 (A) and TAZ‐depleted Kyse‐150 (B) cells 2, 6, 12, and 24 h post‐irradiation. C, D, Correlation between TAZ and NHEJ‐associated genes in esophageal cancer sequencing data. C, Correlation factors of all genes. D, Correlation between TAZ (y‐axis) and 10 TAZ‐correlated NHEJ genes. E, Correlation coefficients between mRNA levels of NHEJ‐associated genes/all the other genes and those of TAZ. F, G, Fold change of mRNA of 12 NHEJ‐associated genes in TAZ‐overexpressing (F) and TAZ‐depleted cells (G) to their corresponding control cells. H, Protein levels of PRKDC, TP53BP1, and XRCC6 in TAZ‐overexpressing cells. Data in C‐E were derived from The Cancer Genome Atlas esophageal cancer mRNA expression data. Data in F and G were derived from RT‐PCR results in vitro. *P < .05, **P < .01, ***P < .001, ****P < .0001. EV, empty vector; FDR, false discovery rate; OE, TAZ‐overexpressing; siScr, scramble siRNA; siTAZ1/2, siRNA against TAZ mRNA1/2; TAZ, plasmid containing TAZ