Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Anti- MUC5B (rabbit polyclonal) |
Santa Cruz |
Cat#
Sc-20119 |
IF (1:2000) |
| Antibody | Anti-MUC5AC (mouse monoclonal) |
Novus Biologicals | Cat # NBP2-15196 |
IF (1:5000) |
| Antibody | anti-β-tubulinIV (mouse monoclonal) | Biogenex | Cat# Mu178-5UC |
IF(1:300) |
| Chemical compound, drug | Methacholine (acetyl-β-methylcholine chloride) |
Sigma | Cat# A2251 |
|
| Chemical compound, drug | CleanCap Cas9 mRNA | TriLink Biotechnologies |
Cat # L-7606 |
20 ng/ul |
| Commercial assay or kit | MEGAshort script T7 Transcription kit | Thermo-fisher | Cat# AM1354 | |
| Commercial assay or kit | KAPA Express Extract Kit Plus amplification module |
KAPA Biosystems | Cat# KK7152 | |
| Commercial assay or kit | MEGAclear Transcription Clean-up kit |
Thermo-fisher | Cat# AM1908 | |
| Commercial assay or kit | QIAquick PCR Purification Kit | Qiagen | Cat# 28104 | |
| Commercial assay or kit | Live/Dead Bacterial Viability Assay | Thermofisher | Cat # L13152 |
|
| Commercial assay or kit | TOPO TA Cloning kit (with PCR 2.1-TOPO vector) | Invitrogen | Cat# K45-0001 |
|
| Gene (Sus scrofa) | EDA1 | Ensembl | Ensembl gene link: ENSSSCG00000021647 | |
| Other | Visualizing media | Invitrogen | 4 nm Nano spheres |
1:10000 |
| Other | Visualizing media | BalTec | Tantalum spheres | 500 μm |
| Other | Visualizing media | Sigma | Tantalum disks | 350 μm |
| Sequence-based reagent | EDA guide 1 | This paper | Guide RNA | GGAATCCCTGGAATCCCTGG |
| Sequence-based reagent | EDA guide 2 | This paper | Guide RNA | GCCCGGTGGTCCCATAACAG |
| Sequence-based reagent | Forward primer | This paper | Primer | gcctgactttgtgttgttagaagtccata |
| Sequence-based reagent | Reverse primer | This paper | Primer | ctgctcttggtatcatgtactcctgatct |
| Software, algorithm | Imaging software | Olympus | CellSens | |
| Software, algorithm | Imaging software | NIH | ImageJ | |
| Software, algorithm | Imaging software | Nikon | NIS Elements | |
| Software, algorithm | Imaging software | Siemens | SOMATON Force | |
| Software, algorithm | Data analysis | GRAPHPAD Software |
GRAPHPAD PRISM |
|
| Software, algorithm | Data analysis | ITK-SNAP | ITK-SNAP | |
| Software, algorithm | Primer-Blast | NCBI | https://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi | |
| Strain, strain background | S. aureus |
Pezzulo et al., 2012
PMID:22763554 |
S. aureus isolate 43SA |