Figure 1.

The expression of H19 is downregulated in HCC cells co‐cultured with Huh7 cells treated with Propofol. (A) The viability of Huh7 cells treated with diverse concentrations of Propofol (0 μmol/L, 5 μmol/L, 25 μmol/L, or 50 μmol/L) was detected by MTT assay. (B and C) MHCC97‐H and HCCLM3 cells were co‐cultured with Huh7 cells treated with Propofol (25 μmol/L) or not. (B) The expression of H19 was measured in MHCC97‐H and HCCLM3 cells by qRT‐PCR. (C) The abundance of metastasis‐associated proteins (Snail, Twist, Vimentin, and E‐cadherin) was determined in the two HCC cells by Western blot assay. *P < .05