Figure 4.

PI16 inhibition increases the sensitivity of HCC xenografts to sorafenib. (A and B) MHCC‐97H cells stably transfected with the PI16 shRNA or empty vector were inoculated subcutaneously in nude mice, and then, treated by sorafenib as described in methods. (C and D) Representative immunohistochemical staining images of the proliferation marker Ki‐67 and quantitative analysis. Scale bar = 20μm. (E and F) Representative immunohistochemical staining images of the apoptosis marker Cleaved caspase‐3 and quantitative analysis. Scale bar = 20μm. (G and H) Representative TUNEL immunofluorescence staining images and quantitative analysis. Scale bar = 20μm. **P < .01, ***P < .001, Student's t test