Fig. 1.

IL-35 expression in DU145, LNCaP, PC-3 and RM-1 cells. a EBI3 and p35 expressions in four PCA cell lines. EBI3 and p35 expression levels were detected by Western blot in DU145, LNCaP, PC-3 and RM-1 cells. b The mRNA levels of EBI3 and p35 in four PCA cell lines. Quantification of EBI3 and p35 mRNA determined by realtime PCR. The mRNA levels of EBI3 and p35 was high in PC-3 cells and RM-1 cells. c Quantification of EBI3, p35, p40 and p28 mRNA in RM-1 cells determined by realtime PCR. The mRNA levels of EBI3 and p35 was high, while mRNA levels of p40 and p28 were low in RM-1 cells