Fig. 5.

G6PD, pSTAT3, and p65 were highly expressed and positively correlated with each other in ccRCC tissues. a, b G6PD mRNA expression level was detected by real-time RT-PCR in 27 ccRCC tumor specimens and adjacent normal tissues. c–f All specimens were subjected to Western blot analysis using anti-G6PD, pSTAT3, or p65 antibodies. The representative Western blot images (c) and the statistics of quantitative analysis were shown (d–f). g–l The correlations between G6PD, pSTAT3, and p65 at the protein level were tested using Pearson correlation analysis. j Immunohistochemistry staining of G6PD, pSTAT3, and p65 in these specimens was conducted, and representative images are shown (200 ×). k Quantification of the staining score from j. Data were expressed as the means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001; ns, nonsignificant vs adjacent normal tissue