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Western blot analysis of protein levels in extracellular vesicles emitted by human ErbB2-positive breast tumor cells. BT-474 (a), BT-474TR (b), MCF7/Her2-18 (c), and HCC-1419 (d) human ErbB2-positive breast cancer cell lines were treated (+) or not (−) with trastuzumab (TZ) for 72 h (a, b, d) or 48 h (c) in 3D culture, and extracellular vesicles emitted by the cells were isolated by ultracentrifugation and analyzed for the levels of the indicated proteins by western blotting
