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. 2020 Oct 7;8:163. doi: 10.1186/s40478-020-01037-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Galectin-3 levels are robustly increased with age in Grn^−/− mouse brain. a, b Representative immunoblot (left) and quantification (right) of galectin-3 levels in the brain lysates of 3- and 18-month-old Grn^+/+ mice and age-matched Grn^−/− mice (n = 4 mice each group). Band values were normalized by total protein values and analyzed by two-way ANOVA. c Levels of mouse brain galectin-3 (ng/mg of protein) in lysates were quantified by ELISA. Galectin-3 levels were normalized to total protein added to assay and results analyzed by two-way ANOVA. d Galectin-3 protein measured in 19-month-old Grn^+/+ and Grn^−/− mice plasma samples by ELISA. Data analyzed using unpaired t-test. e Brain sections from Grn^+/+, Grn^+/−, Grn^−/− mice at 3-, 12- and 24-months of age were immunostained with galectin-3 antibody. Representative images of thalamus from each genotype are shown. f 19-month-old Grn^+/+ and age-matched Grn^−/− mice brain coronal sections were stained with anti-GPNMB antibody and images from multiple regions (cortex (I, VII), hippocampus (II, VIII), corpus callosum (III, IX), striatum (IV, X), thalamus (V, XI) and hypothalamus (VI, XII)) are shown. Scale bars (1 nm to 100 µm) are labeled in images and quantitative data shown as mean ± SEM, p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001