Fig. 5.

Sty knockdown largely rescues the phenotype of Ama depletion. (A) Repo immunofluorescence of third-instar larval brains show a partial increase in size in repo>sty^RNAi Ama^RNAi, and repo>rl^sem Ama^RNAi relative to that in repo>Ama^RNAi. Dashed outlines show brain regions. (B) Quantification of Repo in brains in A reveal that sty knockdown or overexpressing activated ERK in Ama-depleted brains partially rescues the number of glia repo>Ama^RNAi. Z-stacks from repo>+ (n=4), repo>Ama^RNAi (n=4), repo>Ama (n=3), repo>Ama^RNAi (n=4), repo>sty^RNAi Ama^RNAi (n=4), repo>rl^sem Ama^RNAi (n=4) brains were counted and values normalized to the average count in repo>+ (set at 1). Data represents the mean±s.e.m. after normalization. **P<0.01; ***P<0.001; NS, not significant (Student's t-test) to compare each genotype to repo>+ (Table S12). (C) Repo immunofluorescence of eye discs indicates that overexpression of activated ERK in Ama-depleted brains partially rescues glial cell migration in repo>Ama^RNAi eye discs. Scale bars: 20 µm.