Fig. 15.
Freeze-fracture electron micrograph of spinach thylakoids. a The semi-circular structures on the left and the right correspond to fracture face views (labeled EFs and PFs) of grana thylakoids generated by splitting of the membrane bilayers as explained in (b). The fracture faces labeled EFu and Pfu belong to unstacked stroma thylakoids that connect the two adjacent grana stacks. The large EFs particles correspond to PSII-LHCII complexes, and the smaller EFu particles to incomplete PSII complexes. The PFs particles correspond to free LHCII and cytb6f complexes, whereas the PFu particles represent PSI, cytb6f, free LHCII and CF0 complexes. b Schematic diagram illustrating the nomenclature used to label freeze-fracture and freeze-etch images of thylakoid membranes (see a and Fig. 16a). From Staehelin and Arntzen (1983). a Bar 0.2 mm