Fig. 3.

FABP7 regulates caveolin-1 expression through acetylation of histone-H3 lysine-27 on caveolin-1 promoter. a, b ChIP assays and subsequent qPCR for H3K27ac on each caveolin-1 promoter region of WT or FABP7-KO primary cultured astrocytes (a), and mock or FABP7-overexpressed NIH-3T3 cells (b). Mouse IgG was used for negative control. C qPCR analysis for mRNA expression of Cav1, Lpl, Scpep1, Cav2, and Egfr in WT and FABP7-KO astrocytes. d ChIP assays and subsequent qPCR with proximal-1 primer set of Cav1, Lpl, Scpep1, Cav2, and Egfr for the levels of H3K27ac in WT and FABP7-KO astrocytes. Data shown are the means ± s.e.m. and representative of 3 independent experiments. *p < 0.05 versus WT or mock